熱門抗體ADAM10 Antibody由ProSci LLC公司研發生產，產品貨號2051，有0.02 mg和0.1 mg兩種規格可供選擇。欣博盛生物是ProSci LLC公司在國內的授權代理商，負責ProSci LLC公司產品在國內銷售，以及ProSci LLC公司產品售前技術支持。

產品信息

實驗圖例

圖1 ADAM10 KO Validation in MEF Cells

Loading: 10 ug of lysate Antibodies: ADAM10 2051, 1 ug/mL and beta-actin 3779-1301, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.2051 detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).

圖2 Immunohistochemistry Validation of ADAM10 in Human Testis

Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ADAM10 antibody (2051) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

圖3 Immunofluorescence Validation of ADAM10 in Rat Thymus

Immunofluorescent analysis of 4% paraformaldehyde-fixed rat thymus labeling ADAM10 with 2051 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

詳情請聯系ProSci中國代理商——欣博盛生物