DR5（Death Receptor 5）是 TNF 受體超家族成員（TNFRSF10B），是 TRAIL（TNF-Related Apoptosis-Inducing Ligand，腫瘤壞死因子相關凋亡誘導配體）的死亡受體之一。TNF 家族中的某些細胞因子（包括 TNF 和 Fas Ligand）通過其含死亡結構域的受體誘導細胞凋亡。TRAIL/Apo2L 是 TNF 家族的新成員，DR4 是最早被鑒定的 TRAIL 受體，而 DR5 是隨后被多個實驗室獨立鑒定的新型含死亡結構域的 TRAIL 受體，又稱 Apo2、TRAIL-R2、TRICK2 或 KILLER。與 DR4 類似，DR5 轉錄本在正常組織和多種腫瘤細胞中廣泛表達。DR5 結合 TRAIL 并介導 TRAIL 誘導的細胞凋亡。DR5 過表達可誘導凋亡并激活 NF-κB 通路。

DR5 Antibody（目錄號 2019）是ProSci公司生產(chǎn)的一種兔源多克隆抗體，特異性靶向DR5（Death Receptor 5，死亡受體5），也稱TRAIL-R2、TNFRSF10B或KILLER。該抗體以人DR5前體蛋白羧基末端附近20個氨基酸的肽段為免疫原制備，經(jīng)肽柱親和層析純化。可應用于Western Blot、免疫組織化學（IHC-P）、免疫熒光（IF）及 ELISA，已在人、小鼠、大鼠樣本中獲得驗證。該抗體與DR4無交叉反應，特異性優(yōu)良，并經(jīng)過基因敲除（KO）、基因敲降（KD）及調(diào)控表達等多重驗證。如需購買ProSci公司產(chǎn)品，請聯(lián)系ProSci授權代理商欣博盛生物。

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實驗圖例

圖1 KO Validation of DR5 in HCT116 Cells (Han et al., 2015)

Anti-cancer drug, Carfilzomib (CFZ), induced up-regulation of DR5 and the expression of DR5 was not detected in DR5-KO HCT 116 cell line with anti-DR5 antibodies (2019).

圖2 Immunohistochemistry Validation of DR5 in Mouse kidney tissue

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DR5 antibody (2019) at 5μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

圖3 Regulated Expression Validation of DR5 in Thyroid Epithelial Cells (Bretz et al., 2002)

Immunostaining with anti-DR5 antibodies shows high levels of DR5 expression in untreated cells and cells treated with each of the three cytokines alone or TNFalpha combined with IL-1b. In contrast, treatment with both IFNg and TNFalpha or all three cytokines greatly reduces DR5 staining. The reduction in staining appears most significant in cytoplasmic regions while some staining is maintained in or around the nucleus.

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